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201116a maxpar x8 antibody labeling kit  (fluidigm)


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    fluidigm 201116a maxpar x8 antibody labeling kit
    201116a Maxpar X8 Antibody Labeling Kit, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/201116a+maxpar+x8+antibody+labeling+kit/Maxpar+MCP9+Antibody+Labeling+Kit/pmc11228397__mmc2-279-0-8
    Average 93 stars, based on 3 article reviews
    201116a maxpar x8 antibody labeling kit - by Bioz Stars, 2026-09
    93/100 stars

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    Article Title: Multi-omic longitudinal study reveals immune correlates of clinical course among hospitalized COVID-19 patients.
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    Antibody Labeling:

    Article Title: The induction of peripheral trained immunity in the pancreas incites anti-tumor activity to control pancreatic cancer progression
    Article Snippet: Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) solution, pH 7.0 (10x1mL, .5M) MilliporeSigma 626547 HRP-protector peroxidase stabilizer Boca Scientific 222050 Antibody Stabilizer PBS Boca Scientific 131050 Sodium Azide, BioUltra, >99.5%(T) MilliporeSigma 71289 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF ) Sigma-Aldrich D0531 ACK Thermo Fisher Scientific A1049201 Brefeldin A Biolegend 420601 Collagenase I Sigma C9891 DNase Sigma D5025 Endotoxin-free DPBS Sigma-Aldrich TMS-012-A Hyaluronidase Sigma H2126 Matrigel® Basement Membrane Matrix, LDEV-free, 5 mL Corning 356234 Ionomycin Calcium Salt Sigma-Aldrich I0634 PMA Sigma-Aldrich 79346 Fixation Buffer BioLegend 420801 Permeabilization buffer BioLegend 421002 Fluoresbrite® YG Microspheres, Calibration Grade 3.00μm Polysciences 18861-1 3.00μm polystyrene Miroparticle Sigma-Aldrich 79166 MACS Running Buffer Miltenyi Biotec 130-091-221 HEPES Corning 25-060-CI TRIzol Invitrogen 15596018 Poly-L-Lysine Advanced BioMatrix 5048 XenoLight D-Luciferin – K+ Salt Bioluminescent Substrate Perkin Elmer 12799 CD11b MicroBeads, Human and mouse Miltenyi Biotec 130-049-601 N-Acetyl-l-cysteine (NAC) Sigma-Aldrich A7250-5G Lipopolysaccharides from E.coli0111:B4 Sigma L4391-1MG .25% Trypsin 2.21 mM EDTA, 1x [-] sodium bicarbonate Corning 25-053-Cl FBS Atlanta Biologicals Cat #: S11150 Bovine Serum Albumin Cohn Fraction V Powder Equitech0Bio, Inc. Cat # BAC62-1000 Lot #171130-0262 Buprenorphine, hydrochloride, Injection Par Pharmaceutical 10153802 p-SCN-Bn-Deferoxamine Macrocyclics B-705 sodium carbonate VWR BJ34277-1L Oxalic acid, anhydrous Sigma 756888-50G UltraPureTM 1 M Tris-HCI Buffer, pH 7.5 Invitrogen 15567027 Zr-89 oxalate Washington University at St. Louis Radionuclide Processing Laboratory Dulbecco’s Phosphate Buffered Saline Sigma D8537 Chloroform Sigma C2432 2-propanol Fisher Scientific A41701 UltaPureTM Glycogen Thermo Fisher Scientific 10814010 iScriptTM cDNA Synthesis Kit BioRad 1708891 iQTM SYBR® Green Supermix BioRad 1708880 RPMI-1640 medium Sigma R8758-1L DMEM- high glucose Sigma D5796-500ML .. MaxPar MCP9 Antibody Labeling Kit, 106Cd Fluidigm Cat # 201106A MaxPar MCP9 Antibody Labeling Kit, 111Cd Fluidigm Cat # 201111A MaxPar MCP9 Antibody Labeling Kit, 112Cd Fluidigm Cat # 201112A MaxPar MCP9 Antibody Labeling Kit, 113Cd Fluidigm Cat # 201113A MaxPar MCP9 Antibody Labeling Kit, 114Cd Fluidigm Cat # 201114A MaxPar MCP9 Antibody Labeling Kit, 116Cd Fluidigm Cat # 201116A MaxPar X8 Antibody Labeling Kit, 146Nd Fluidigm Cat # 201146A MaxPar X8 Antibody Labeling Kit, 149 Sm Fluidigm Cat # 201149A MaxPar X8 Antibody Labeling Kit, 154 Sm Fluidigm Cat # 201154A MaxPar X8 Antibody Labeling Kit, 156 Gd Fluidigm Cat # 201156A MaxPar X8 Antibody Labeling Kit, 162 Dy Fluidigm Cat # 201162A MaxPar X8 Antibody Labeling Kit, 165 Ho Fluidigm Cat # 201165A MaxPar X8 Antibody Labeling Kit, 166 Er Fluidigm Cat # 201166A MaxPar X8 Antibody Labeling Kit, 168Er Fluidigm Cat # 201168A MaxPar X8 Antibody Labeling Kit, 173Yb Fluidigm Cat # 201173A ELISA MAXTM standard set Mouse TNF-a BioLegend 430901 ELISA MAXTM standard set Mouse IL-6 BioLegend 431301 Mouse MIF DuoSet ELISA R&D Systems DY1978 Invitrogen pHrodoTM Green S. aureus BioParticlesTM Phagocytosis Kit for Flow cytometry Thermo Fisher Scientific P535367 Amylase Activity Assay Kit Millipore Sigma MAK009 QIAGEN RNAeasy Kit QIAGEN 74104 Promega Luciferase Assay Systems Luciferase Assay w/ Reporter Lysis buffer Promega E4030 Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1, 4 rxns 10X Genomics PN-1000128 MiSeq Reagent Micro Kit V2 300 cycles Illumina MS-102-2002 NextSeq 500 High Output Kit v2.5 (150 cycles) Illumina 20024907 .. KPC Cell Line Ximbio 153474 Pan02 Cell Line Gift from Dr. Yong Lu at Wake Forest University KPC/FC1242 Luc/GFP clone 3 Gift from Dr. Michael Dwinell, PhD at the Medical College of Wisconsin Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory 000664 Mouse: B6.129S4-Ccr2 tm1I fc/J (CCR2 -/-) The Jackson Laboratory 004999 Mouse: Dectin-1 -/- Bred at the University of Louisville (Saijo et al, 2007) Mouse: NOD.Cg-Prkdcsc id Il2rg tm1Wjl/SzJ (NSG) The Jackson Laboratory 005557 Mouse: B6(Cg)-Tyr c-2J (B6 Albino mice) Gift from Dr. Warawa and bred at the University of Louisville

    Article Title: CD37 is a safe chimeric antigen receptor target to treat acute myeloid leukemia
    Article Snippet: 201113A Maxpar MCP9 Antibody Labeling Kit, 166Cd–4 Rxn Standard BioTools Cat.No. .. 201116A Maxpar X8 Antibody Labeling Kit, 145Nd—4 Rxn Standard BioTools Cat.No. .. 201145A Maxpar X8 Antibody Labeling Kit, 146Nd—4 Rxn Standard BioTools Cat.No.

    Article Title: CD37 is a safe chimeric antigen receptor target to treat acute myeloid leukemia.
    Article Snippet: 201113A Maxpar MCP9 Antibody Labeling Kit, 166Cd–4 Rxn Standard BioTools Cat.No. .. 201116A Maxpar X8 Antibody Labeling Kit, 145Nd—4 Rxn Standard BioTools Cat.No. .. 201145A Maxpar X8 Antibody Labeling Kit, 146Nd—4 Rxn Standard BioTools Cat.No.

    Enzyme-linked Immunosorbent Assay:

    Article Title: The induction of peripheral trained immunity in the pancreas incites anti-tumor activity to control pancreatic cancer progression
    Article Snippet: Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) solution, pH 7.0 (10x1mL, .5M) MilliporeSigma 626547 HRP-protector peroxidase stabilizer Boca Scientific 222050 Antibody Stabilizer PBS Boca Scientific 131050 Sodium Azide, BioUltra, >99.5%(T) MilliporeSigma 71289 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF ) Sigma-Aldrich D0531 ACK Thermo Fisher Scientific A1049201 Brefeldin A Biolegend 420601 Collagenase I Sigma C9891 DNase Sigma D5025 Endotoxin-free DPBS Sigma-Aldrich TMS-012-A Hyaluronidase Sigma H2126 Matrigel® Basement Membrane Matrix, LDEV-free, 5 mL Corning 356234 Ionomycin Calcium Salt Sigma-Aldrich I0634 PMA Sigma-Aldrich 79346 Fixation Buffer BioLegend 420801 Permeabilization buffer BioLegend 421002 Fluoresbrite® YG Microspheres, Calibration Grade 3.00μm Polysciences 18861-1 3.00μm polystyrene Miroparticle Sigma-Aldrich 79166 MACS Running Buffer Miltenyi Biotec 130-091-221 HEPES Corning 25-060-CI TRIzol Invitrogen 15596018 Poly-L-Lysine Advanced BioMatrix 5048 XenoLight D-Luciferin – K+ Salt Bioluminescent Substrate Perkin Elmer 12799 CD11b MicroBeads, Human and mouse Miltenyi Biotec 130-049-601 N-Acetyl-l-cysteine (NAC) Sigma-Aldrich A7250-5G Lipopolysaccharides from E.coli0111:B4 Sigma L4391-1MG .25% Trypsin 2.21 mM EDTA, 1x [-] sodium bicarbonate Corning 25-053-Cl FBS Atlanta Biologicals Cat #: S11150 Bovine Serum Albumin Cohn Fraction V Powder Equitech0Bio, Inc. Cat # BAC62-1000 Lot #171130-0262 Buprenorphine, hydrochloride, Injection Par Pharmaceutical 10153802 p-SCN-Bn-Deferoxamine Macrocyclics B-705 sodium carbonate VWR BJ34277-1L Oxalic acid, anhydrous Sigma 756888-50G UltraPureTM 1 M Tris-HCI Buffer, pH 7.5 Invitrogen 15567027 Zr-89 oxalate Washington University at St. Louis Radionuclide Processing Laboratory Dulbecco’s Phosphate Buffered Saline Sigma D8537 Chloroform Sigma C2432 2-propanol Fisher Scientific A41701 UltaPureTM Glycogen Thermo Fisher Scientific 10814010 iScriptTM cDNA Synthesis Kit BioRad 1708891 iQTM SYBR® Green Supermix BioRad 1708880 RPMI-1640 medium Sigma R8758-1L DMEM- high glucose Sigma D5796-500ML .. MaxPar MCP9 Antibody Labeling Kit, 106Cd Fluidigm Cat # 201106A MaxPar MCP9 Antibody Labeling Kit, 111Cd Fluidigm Cat # 201111A MaxPar MCP9 Antibody Labeling Kit, 112Cd Fluidigm Cat # 201112A MaxPar MCP9 Antibody Labeling Kit, 113Cd Fluidigm Cat # 201113A MaxPar MCP9 Antibody Labeling Kit, 114Cd Fluidigm Cat # 201114A MaxPar MCP9 Antibody Labeling Kit, 116Cd Fluidigm Cat # 201116A MaxPar X8 Antibody Labeling Kit, 146Nd Fluidigm Cat # 201146A MaxPar X8 Antibody Labeling Kit, 149 Sm Fluidigm Cat # 201149A MaxPar X8 Antibody Labeling Kit, 154 Sm Fluidigm Cat # 201154A MaxPar X8 Antibody Labeling Kit, 156 Gd Fluidigm Cat # 201156A MaxPar X8 Antibody Labeling Kit, 162 Dy Fluidigm Cat # 201162A MaxPar X8 Antibody Labeling Kit, 165 Ho Fluidigm Cat # 201165A MaxPar X8 Antibody Labeling Kit, 166 Er Fluidigm Cat # 201166A MaxPar X8 Antibody Labeling Kit, 168Er Fluidigm Cat # 201168A MaxPar X8 Antibody Labeling Kit, 173Yb Fluidigm Cat # 201173A ELISA MAXTM standard set Mouse TNF-a BioLegend 430901 ELISA MAXTM standard set Mouse IL-6 BioLegend 431301 Mouse MIF DuoSet ELISA R&D Systems DY1978 Invitrogen pHrodoTM Green S. aureus BioParticlesTM Phagocytosis Kit for Flow cytometry Thermo Fisher Scientific P535367 Amylase Activity Assay Kit Millipore Sigma MAK009 QIAGEN RNAeasy Kit QIAGEN 74104 Promega Luciferase Assay Systems Luciferase Assay w/ Reporter Lysis buffer Promega E4030 Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1, 4 rxns 10X Genomics PN-1000128 MiSeq Reagent Micro Kit V2 300 cycles Illumina MS-102-2002 NextSeq 500 High Output Kit v2.5 (150 cycles) Illumina 20024907 .. KPC Cell Line Ximbio 153474 Pan02 Cell Line Gift from Dr. Yong Lu at Wake Forest University KPC/FC1242 Luc/GFP clone 3 Gift from Dr. Michael Dwinell, PhD at the Medical College of Wisconsin Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory 000664 Mouse: B6.129S4-Ccr2 tm1I fc/J (CCR2 -/-) The Jackson Laboratory 004999 Mouse: Dectin-1 -/- Bred at the University of Louisville (Saijo et al, 2007) Mouse: NOD.Cg-Prkdcsc id Il2rg tm1Wjl/SzJ (NSG) The Jackson Laboratory 005557 Mouse: B6(Cg)-Tyr c-2J (B6 Albino mice) Gift from Dr. Warawa and bred at the University of Louisville

    Flow Cytometry:

    Article Title: The induction of peripheral trained immunity in the pancreas incites anti-tumor activity to control pancreatic cancer progression
    Article Snippet: Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) solution, pH 7.0 (10x1mL, .5M) MilliporeSigma 626547 HRP-protector peroxidase stabilizer Boca Scientific 222050 Antibody Stabilizer PBS Boca Scientific 131050 Sodium Azide, BioUltra, >99.5%(T) MilliporeSigma 71289 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF ) Sigma-Aldrich D0531 ACK Thermo Fisher Scientific A1049201 Brefeldin A Biolegend 420601 Collagenase I Sigma C9891 DNase Sigma D5025 Endotoxin-free DPBS Sigma-Aldrich TMS-012-A Hyaluronidase Sigma H2126 Matrigel® Basement Membrane Matrix, LDEV-free, 5 mL Corning 356234 Ionomycin Calcium Salt Sigma-Aldrich I0634 PMA Sigma-Aldrich 79346 Fixation Buffer BioLegend 420801 Permeabilization buffer BioLegend 421002 Fluoresbrite® YG Microspheres, Calibration Grade 3.00μm Polysciences 18861-1 3.00μm polystyrene Miroparticle Sigma-Aldrich 79166 MACS Running Buffer Miltenyi Biotec 130-091-221 HEPES Corning 25-060-CI TRIzol Invitrogen 15596018 Poly-L-Lysine Advanced BioMatrix 5048 XenoLight D-Luciferin – K+ Salt Bioluminescent Substrate Perkin Elmer 12799 CD11b MicroBeads, Human and mouse Miltenyi Biotec 130-049-601 N-Acetyl-l-cysteine (NAC) Sigma-Aldrich A7250-5G Lipopolysaccharides from E.coli0111:B4 Sigma L4391-1MG .25% Trypsin 2.21 mM EDTA, 1x [-] sodium bicarbonate Corning 25-053-Cl FBS Atlanta Biologicals Cat #: S11150 Bovine Serum Albumin Cohn Fraction V Powder Equitech0Bio, Inc. Cat # BAC62-1000 Lot #171130-0262 Buprenorphine, hydrochloride, Injection Par Pharmaceutical 10153802 p-SCN-Bn-Deferoxamine Macrocyclics B-705 sodium carbonate VWR BJ34277-1L Oxalic acid, anhydrous Sigma 756888-50G UltraPureTM 1 M Tris-HCI Buffer, pH 7.5 Invitrogen 15567027 Zr-89 oxalate Washington University at St. Louis Radionuclide Processing Laboratory Dulbecco’s Phosphate Buffered Saline Sigma D8537 Chloroform Sigma C2432 2-propanol Fisher Scientific A41701 UltaPureTM Glycogen Thermo Fisher Scientific 10814010 iScriptTM cDNA Synthesis Kit BioRad 1708891 iQTM SYBR® Green Supermix BioRad 1708880 RPMI-1640 medium Sigma R8758-1L DMEM- high glucose Sigma D5796-500ML .. MaxPar MCP9 Antibody Labeling Kit, 106Cd Fluidigm Cat # 201106A MaxPar MCP9 Antibody Labeling Kit, 111Cd Fluidigm Cat # 201111A MaxPar MCP9 Antibody Labeling Kit, 112Cd Fluidigm Cat # 201112A MaxPar MCP9 Antibody Labeling Kit, 113Cd Fluidigm Cat # 201113A MaxPar MCP9 Antibody Labeling Kit, 114Cd Fluidigm Cat # 201114A MaxPar MCP9 Antibody Labeling Kit, 116Cd Fluidigm Cat # 201116A MaxPar X8 Antibody Labeling Kit, 146Nd Fluidigm Cat # 201146A MaxPar X8 Antibody Labeling Kit, 149 Sm Fluidigm Cat # 201149A MaxPar X8 Antibody Labeling Kit, 154 Sm Fluidigm Cat # 201154A MaxPar X8 Antibody Labeling Kit, 156 Gd Fluidigm Cat # 201156A MaxPar X8 Antibody Labeling Kit, 162 Dy Fluidigm Cat # 201162A MaxPar X8 Antibody Labeling Kit, 165 Ho Fluidigm Cat # 201165A MaxPar X8 Antibody Labeling Kit, 166 Er Fluidigm Cat # 201166A MaxPar X8 Antibody Labeling Kit, 168Er Fluidigm Cat # 201168A MaxPar X8 Antibody Labeling Kit, 173Yb Fluidigm Cat # 201173A ELISA MAXTM standard set Mouse TNF-a BioLegend 430901 ELISA MAXTM standard set Mouse IL-6 BioLegend 431301 Mouse MIF DuoSet ELISA R&D Systems DY1978 Invitrogen pHrodoTM Green S. aureus BioParticlesTM Phagocytosis Kit for Flow cytometry Thermo Fisher Scientific P535367 Amylase Activity Assay Kit Millipore Sigma MAK009 QIAGEN RNAeasy Kit QIAGEN 74104 Promega Luciferase Assay Systems Luciferase Assay w/ Reporter Lysis buffer Promega E4030 Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1, 4 rxns 10X Genomics PN-1000128 MiSeq Reagent Micro Kit V2 300 cycles Illumina MS-102-2002 NextSeq 500 High Output Kit v2.5 (150 cycles) Illumina 20024907 .. KPC Cell Line Ximbio 153474 Pan02 Cell Line Gift from Dr. Yong Lu at Wake Forest University KPC/FC1242 Luc/GFP clone 3 Gift from Dr. Michael Dwinell, PhD at the Medical College of Wisconsin Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory 000664 Mouse: B6.129S4-Ccr2 tm1I fc/J (CCR2 -/-) The Jackson Laboratory 004999 Mouse: Dectin-1 -/- Bred at the University of Louisville (Saijo et al, 2007) Mouse: NOD.Cg-Prkdcsc id Il2rg tm1Wjl/SzJ (NSG) The Jackson Laboratory 005557 Mouse: B6(Cg)-Tyr c-2J (B6 Albino mice) Gift from Dr. Warawa and bred at the University of Louisville

    Activity Assay:

    Article Title: The induction of peripheral trained immunity in the pancreas incites anti-tumor activity to control pancreatic cancer progression
    Article Snippet: Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) solution, pH 7.0 (10x1mL, .5M) MilliporeSigma 626547 HRP-protector peroxidase stabilizer Boca Scientific 222050 Antibody Stabilizer PBS Boca Scientific 131050 Sodium Azide, BioUltra, >99.5%(T) MilliporeSigma 71289 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF ) Sigma-Aldrich D0531 ACK Thermo Fisher Scientific A1049201 Brefeldin A Biolegend 420601 Collagenase I Sigma C9891 DNase Sigma D5025 Endotoxin-free DPBS Sigma-Aldrich TMS-012-A Hyaluronidase Sigma H2126 Matrigel® Basement Membrane Matrix, LDEV-free, 5 mL Corning 356234 Ionomycin Calcium Salt Sigma-Aldrich I0634 PMA Sigma-Aldrich 79346 Fixation Buffer BioLegend 420801 Permeabilization buffer BioLegend 421002 Fluoresbrite® YG Microspheres, Calibration Grade 3.00μm Polysciences 18861-1 3.00μm polystyrene Miroparticle Sigma-Aldrich 79166 MACS Running Buffer Miltenyi Biotec 130-091-221 HEPES Corning 25-060-CI TRIzol Invitrogen 15596018 Poly-L-Lysine Advanced BioMatrix 5048 XenoLight D-Luciferin – K+ Salt Bioluminescent Substrate Perkin Elmer 12799 CD11b MicroBeads, Human and mouse Miltenyi Biotec 130-049-601 N-Acetyl-l-cysteine (NAC) Sigma-Aldrich A7250-5G Lipopolysaccharides from E.coli0111:B4 Sigma L4391-1MG .25% Trypsin 2.21 mM EDTA, 1x [-] sodium bicarbonate Corning 25-053-Cl FBS Atlanta Biologicals Cat #: S11150 Bovine Serum Albumin Cohn Fraction V Powder Equitech0Bio, Inc. Cat # BAC62-1000 Lot #171130-0262 Buprenorphine, hydrochloride, Injection Par Pharmaceutical 10153802 p-SCN-Bn-Deferoxamine Macrocyclics B-705 sodium carbonate VWR BJ34277-1L Oxalic acid, anhydrous Sigma 756888-50G UltraPureTM 1 M Tris-HCI Buffer, pH 7.5 Invitrogen 15567027 Zr-89 oxalate Washington University at St. Louis Radionuclide Processing Laboratory Dulbecco’s Phosphate Buffered Saline Sigma D8537 Chloroform Sigma C2432 2-propanol Fisher Scientific A41701 UltaPureTM Glycogen Thermo Fisher Scientific 10814010 iScriptTM cDNA Synthesis Kit BioRad 1708891 iQTM SYBR® Green Supermix BioRad 1708880 RPMI-1640 medium Sigma R8758-1L DMEM- high glucose Sigma D5796-500ML .. MaxPar MCP9 Antibody Labeling Kit, 106Cd Fluidigm Cat # 201106A MaxPar MCP9 Antibody Labeling Kit, 111Cd Fluidigm Cat # 201111A MaxPar MCP9 Antibody Labeling Kit, 112Cd Fluidigm Cat # 201112A MaxPar MCP9 Antibody Labeling Kit, 113Cd Fluidigm Cat # 201113A MaxPar MCP9 Antibody Labeling Kit, 114Cd Fluidigm Cat # 201114A MaxPar MCP9 Antibody Labeling Kit, 116Cd Fluidigm Cat # 201116A MaxPar X8 Antibody Labeling Kit, 146Nd Fluidigm Cat # 201146A MaxPar X8 Antibody Labeling Kit, 149 Sm Fluidigm Cat # 201149A MaxPar X8 Antibody Labeling Kit, 154 Sm Fluidigm Cat # 201154A MaxPar X8 Antibody Labeling Kit, 156 Gd Fluidigm Cat # 201156A MaxPar X8 Antibody Labeling Kit, 162 Dy Fluidigm Cat # 201162A MaxPar X8 Antibody Labeling Kit, 165 Ho Fluidigm Cat # 201165A MaxPar X8 Antibody Labeling Kit, 166 Er Fluidigm Cat # 201166A MaxPar X8 Antibody Labeling Kit, 168Er Fluidigm Cat # 201168A MaxPar X8 Antibody Labeling Kit, 173Yb Fluidigm Cat # 201173A ELISA MAXTM standard set Mouse TNF-a BioLegend 430901 ELISA MAXTM standard set Mouse IL-6 BioLegend 431301 Mouse MIF DuoSet ELISA R&D Systems DY1978 Invitrogen pHrodoTM Green S. aureus BioParticlesTM Phagocytosis Kit for Flow cytometry Thermo Fisher Scientific P535367 Amylase Activity Assay Kit Millipore Sigma MAK009 QIAGEN RNAeasy Kit QIAGEN 74104 Promega Luciferase Assay Systems Luciferase Assay w/ Reporter Lysis buffer Promega E4030 Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1, 4 rxns 10X Genomics PN-1000128 MiSeq Reagent Micro Kit V2 300 cycles Illumina MS-102-2002 NextSeq 500 High Output Kit v2.5 (150 cycles) Illumina 20024907 .. KPC Cell Line Ximbio 153474 Pan02 Cell Line Gift from Dr. Yong Lu at Wake Forest University KPC/FC1242 Luc/GFP clone 3 Gift from Dr. Michael Dwinell, PhD at the Medical College of Wisconsin Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory 000664 Mouse: B6.129S4-Ccr2 tm1I fc/J (CCR2 -/-) The Jackson Laboratory 004999 Mouse: Dectin-1 -/- Bred at the University of Louisville (Saijo et al, 2007) Mouse: NOD.Cg-Prkdcsc id Il2rg tm1Wjl/SzJ (NSG) The Jackson Laboratory 005557 Mouse: B6(Cg)-Tyr c-2J (B6 Albino mice) Gift from Dr. Warawa and bred at the University of Louisville

    Luciferase:

    Article Title: The induction of peripheral trained immunity in the pancreas incites anti-tumor activity to control pancreatic cancer progression
    Article Snippet: Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) solution, pH 7.0 (10x1mL, .5M) MilliporeSigma 626547 HRP-protector peroxidase stabilizer Boca Scientific 222050 Antibody Stabilizer PBS Boca Scientific 131050 Sodium Azide, BioUltra, >99.5%(T) MilliporeSigma 71289 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF ) Sigma-Aldrich D0531 ACK Thermo Fisher Scientific A1049201 Brefeldin A Biolegend 420601 Collagenase I Sigma C9891 DNase Sigma D5025 Endotoxin-free DPBS Sigma-Aldrich TMS-012-A Hyaluronidase Sigma H2126 Matrigel® Basement Membrane Matrix, LDEV-free, 5 mL Corning 356234 Ionomycin Calcium Salt Sigma-Aldrich I0634 PMA Sigma-Aldrich 79346 Fixation Buffer BioLegend 420801 Permeabilization buffer BioLegend 421002 Fluoresbrite® YG Microspheres, Calibration Grade 3.00μm Polysciences 18861-1 3.00μm polystyrene Miroparticle Sigma-Aldrich 79166 MACS Running Buffer Miltenyi Biotec 130-091-221 HEPES Corning 25-060-CI TRIzol Invitrogen 15596018 Poly-L-Lysine Advanced BioMatrix 5048 XenoLight D-Luciferin – K+ Salt Bioluminescent Substrate Perkin Elmer 12799 CD11b MicroBeads, Human and mouse Miltenyi Biotec 130-049-601 N-Acetyl-l-cysteine (NAC) Sigma-Aldrich A7250-5G Lipopolysaccharides from E.coli0111:B4 Sigma L4391-1MG .25% Trypsin 2.21 mM EDTA, 1x [-] sodium bicarbonate Corning 25-053-Cl FBS Atlanta Biologicals Cat #: S11150 Bovine Serum Albumin Cohn Fraction V Powder Equitech0Bio, Inc. Cat # BAC62-1000 Lot #171130-0262 Buprenorphine, hydrochloride, Injection Par Pharmaceutical 10153802 p-SCN-Bn-Deferoxamine Macrocyclics B-705 sodium carbonate VWR BJ34277-1L Oxalic acid, anhydrous Sigma 756888-50G UltraPureTM 1 M Tris-HCI Buffer, pH 7.5 Invitrogen 15567027 Zr-89 oxalate Washington University at St. Louis Radionuclide Processing Laboratory Dulbecco’s Phosphate Buffered Saline Sigma D8537 Chloroform Sigma C2432 2-propanol Fisher Scientific A41701 UltaPureTM Glycogen Thermo Fisher Scientific 10814010 iScriptTM cDNA Synthesis Kit BioRad 1708891 iQTM SYBR® Green Supermix BioRad 1708880 RPMI-1640 medium Sigma R8758-1L DMEM- high glucose Sigma D5796-500ML .. MaxPar MCP9 Antibody Labeling Kit, 106Cd Fluidigm Cat # 201106A MaxPar MCP9 Antibody Labeling Kit, 111Cd Fluidigm Cat # 201111A MaxPar MCP9 Antibody Labeling Kit, 112Cd Fluidigm Cat # 201112A MaxPar MCP9 Antibody Labeling Kit, 113Cd Fluidigm Cat # 201113A MaxPar MCP9 Antibody Labeling Kit, 114Cd Fluidigm Cat # 201114A MaxPar MCP9 Antibody Labeling Kit, 116Cd Fluidigm Cat # 201116A MaxPar X8 Antibody Labeling Kit, 146Nd Fluidigm Cat # 201146A MaxPar X8 Antibody Labeling Kit, 149 Sm Fluidigm Cat # 201149A MaxPar X8 Antibody Labeling Kit, 154 Sm Fluidigm Cat # 201154A MaxPar X8 Antibody Labeling Kit, 156 Gd Fluidigm Cat # 201156A MaxPar X8 Antibody Labeling Kit, 162 Dy Fluidigm Cat # 201162A MaxPar X8 Antibody Labeling Kit, 165 Ho Fluidigm Cat # 201165A MaxPar X8 Antibody Labeling Kit, 166 Er Fluidigm Cat # 201166A MaxPar X8 Antibody Labeling Kit, 168Er Fluidigm Cat # 201168A MaxPar X8 Antibody Labeling Kit, 173Yb Fluidigm Cat # 201173A ELISA MAXTM standard set Mouse TNF-a BioLegend 430901 ELISA MAXTM standard set Mouse IL-6 BioLegend 431301 Mouse MIF DuoSet ELISA R&D Systems DY1978 Invitrogen pHrodoTM Green S. aureus BioParticlesTM Phagocytosis Kit for Flow cytometry Thermo Fisher Scientific P535367 Amylase Activity Assay Kit Millipore Sigma MAK009 QIAGEN RNAeasy Kit QIAGEN 74104 Promega Luciferase Assay Systems Luciferase Assay w/ Reporter Lysis buffer Promega E4030 Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1, 4 rxns 10X Genomics PN-1000128 MiSeq Reagent Micro Kit V2 300 cycles Illumina MS-102-2002 NextSeq 500 High Output Kit v2.5 (150 cycles) Illumina 20024907 .. KPC Cell Line Ximbio 153474 Pan02 Cell Line Gift from Dr. Yong Lu at Wake Forest University KPC/FC1242 Luc/GFP clone 3 Gift from Dr. Michael Dwinell, PhD at the Medical College of Wisconsin Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory 000664 Mouse: B6.129S4-Ccr2 tm1I fc/J (CCR2 -/-) The Jackson Laboratory 004999 Mouse: Dectin-1 -/- Bred at the University of Louisville (Saijo et al, 2007) Mouse: NOD.Cg-Prkdcsc id Il2rg tm1Wjl/SzJ (NSG) The Jackson Laboratory 005557 Mouse: B6(Cg)-Tyr c-2J (B6 Albino mice) Gift from Dr. Warawa and bred at the University of Louisville

    Lysis:

    Article Title: The induction of peripheral trained immunity in the pancreas incites anti-tumor activity to control pancreatic cancer progression
    Article Snippet: Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) solution, pH 7.0 (10x1mL, .5M) MilliporeSigma 626547 HRP-protector peroxidase stabilizer Boca Scientific 222050 Antibody Stabilizer PBS Boca Scientific 131050 Sodium Azide, BioUltra, >99.5%(T) MilliporeSigma 71289 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF ) Sigma-Aldrich D0531 ACK Thermo Fisher Scientific A1049201 Brefeldin A Biolegend 420601 Collagenase I Sigma C9891 DNase Sigma D5025 Endotoxin-free DPBS Sigma-Aldrich TMS-012-A Hyaluronidase Sigma H2126 Matrigel® Basement Membrane Matrix, LDEV-free, 5 mL Corning 356234 Ionomycin Calcium Salt Sigma-Aldrich I0634 PMA Sigma-Aldrich 79346 Fixation Buffer BioLegend 420801 Permeabilization buffer BioLegend 421002 Fluoresbrite® YG Microspheres, Calibration Grade 3.00μm Polysciences 18861-1 3.00μm polystyrene Miroparticle Sigma-Aldrich 79166 MACS Running Buffer Miltenyi Biotec 130-091-221 HEPES Corning 25-060-CI TRIzol Invitrogen 15596018 Poly-L-Lysine Advanced BioMatrix 5048 XenoLight D-Luciferin – K+ Salt Bioluminescent Substrate Perkin Elmer 12799 CD11b MicroBeads, Human and mouse Miltenyi Biotec 130-049-601 N-Acetyl-l-cysteine (NAC) Sigma-Aldrich A7250-5G Lipopolysaccharides from E.coli0111:B4 Sigma L4391-1MG .25% Trypsin 2.21 mM EDTA, 1x [-] sodium bicarbonate Corning 25-053-Cl FBS Atlanta Biologicals Cat #: S11150 Bovine Serum Albumin Cohn Fraction V Powder Equitech0Bio, Inc. Cat # BAC62-1000 Lot #171130-0262 Buprenorphine, hydrochloride, Injection Par Pharmaceutical 10153802 p-SCN-Bn-Deferoxamine Macrocyclics B-705 sodium carbonate VWR BJ34277-1L Oxalic acid, anhydrous Sigma 756888-50G UltraPureTM 1 M Tris-HCI Buffer, pH 7.5 Invitrogen 15567027 Zr-89 oxalate Washington University at St. Louis Radionuclide Processing Laboratory Dulbecco’s Phosphate Buffered Saline Sigma D8537 Chloroform Sigma C2432 2-propanol Fisher Scientific A41701 UltaPureTM Glycogen Thermo Fisher Scientific 10814010 iScriptTM cDNA Synthesis Kit BioRad 1708891 iQTM SYBR® Green Supermix BioRad 1708880 RPMI-1640 medium Sigma R8758-1L DMEM- high glucose Sigma D5796-500ML .. MaxPar MCP9 Antibody Labeling Kit, 106Cd Fluidigm Cat # 201106A MaxPar MCP9 Antibody Labeling Kit, 111Cd Fluidigm Cat # 201111A MaxPar MCP9 Antibody Labeling Kit, 112Cd Fluidigm Cat # 201112A MaxPar MCP9 Antibody Labeling Kit, 113Cd Fluidigm Cat # 201113A MaxPar MCP9 Antibody Labeling Kit, 114Cd Fluidigm Cat # 201114A MaxPar MCP9 Antibody Labeling Kit, 116Cd Fluidigm Cat # 201116A MaxPar X8 Antibody Labeling Kit, 146Nd Fluidigm Cat # 201146A MaxPar X8 Antibody Labeling Kit, 149 Sm Fluidigm Cat # 201149A MaxPar X8 Antibody Labeling Kit, 154 Sm Fluidigm Cat # 201154A MaxPar X8 Antibody Labeling Kit, 156 Gd Fluidigm Cat # 201156A MaxPar X8 Antibody Labeling Kit, 162 Dy Fluidigm Cat # 201162A MaxPar X8 Antibody Labeling Kit, 165 Ho Fluidigm Cat # 201165A MaxPar X8 Antibody Labeling Kit, 166 Er Fluidigm Cat # 201166A MaxPar X8 Antibody Labeling Kit, 168Er Fluidigm Cat # 201168A MaxPar X8 Antibody Labeling Kit, 173Yb Fluidigm Cat # 201173A ELISA MAXTM standard set Mouse TNF-a BioLegend 430901 ELISA MAXTM standard set Mouse IL-6 BioLegend 431301 Mouse MIF DuoSet ELISA R&D Systems DY1978 Invitrogen pHrodoTM Green S. aureus BioParticlesTM Phagocytosis Kit for Flow cytometry Thermo Fisher Scientific P535367 Amylase Activity Assay Kit Millipore Sigma MAK009 QIAGEN RNAeasy Kit QIAGEN 74104 Promega Luciferase Assay Systems Luciferase Assay w/ Reporter Lysis buffer Promega E4030 Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1, 4 rxns 10X Genomics PN-1000128 MiSeq Reagent Micro Kit V2 300 cycles Illumina MS-102-2002 NextSeq 500 High Output Kit v2.5 (150 cycles) Illumina 20024907 .. KPC Cell Line Ximbio 153474 Pan02 Cell Line Gift from Dr. Yong Lu at Wake Forest University KPC/FC1242 Luc/GFP clone 3 Gift from Dr. Michael Dwinell, PhD at the Medical College of Wisconsin Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory 000664 Mouse: B6.129S4-Ccr2 tm1I fc/J (CCR2 -/-) The Jackson Laboratory 004999 Mouse: Dectin-1 -/- Bred at the University of Louisville (Saijo et al, 2007) Mouse: NOD.Cg-Prkdcsc id Il2rg tm1Wjl/SzJ (NSG) The Jackson Laboratory 005557 Mouse: B6(Cg)-Tyr c-2J (B6 Albino mice) Gift from Dr. Warawa and bred at the University of Louisville

    Single Cell:

    Article Title: The induction of peripheral trained immunity in the pancreas incites anti-tumor activity to control pancreatic cancer progression
    Article Snippet: Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) solution, pH 7.0 (10x1mL, .5M) MilliporeSigma 626547 HRP-protector peroxidase stabilizer Boca Scientific 222050 Antibody Stabilizer PBS Boca Scientific 131050 Sodium Azide, BioUltra, >99.5%(T) MilliporeSigma 71289 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF ) Sigma-Aldrich D0531 ACK Thermo Fisher Scientific A1049201 Brefeldin A Biolegend 420601 Collagenase I Sigma C9891 DNase Sigma D5025 Endotoxin-free DPBS Sigma-Aldrich TMS-012-A Hyaluronidase Sigma H2126 Matrigel® Basement Membrane Matrix, LDEV-free, 5 mL Corning 356234 Ionomycin Calcium Salt Sigma-Aldrich I0634 PMA Sigma-Aldrich 79346 Fixation Buffer BioLegend 420801 Permeabilization buffer BioLegend 421002 Fluoresbrite® YG Microspheres, Calibration Grade 3.00μm Polysciences 18861-1 3.00μm polystyrene Miroparticle Sigma-Aldrich 79166 MACS Running Buffer Miltenyi Biotec 130-091-221 HEPES Corning 25-060-CI TRIzol Invitrogen 15596018 Poly-L-Lysine Advanced BioMatrix 5048 XenoLight D-Luciferin – K+ Salt Bioluminescent Substrate Perkin Elmer 12799 CD11b MicroBeads, Human and mouse Miltenyi Biotec 130-049-601 N-Acetyl-l-cysteine (NAC) Sigma-Aldrich A7250-5G Lipopolysaccharides from E.coli0111:B4 Sigma L4391-1MG .25% Trypsin 2.21 mM EDTA, 1x [-] sodium bicarbonate Corning 25-053-Cl FBS Atlanta Biologicals Cat #: S11150 Bovine Serum Albumin Cohn Fraction V Powder Equitech0Bio, Inc. Cat # BAC62-1000 Lot #171130-0262 Buprenorphine, hydrochloride, Injection Par Pharmaceutical 10153802 p-SCN-Bn-Deferoxamine Macrocyclics B-705 sodium carbonate VWR BJ34277-1L Oxalic acid, anhydrous Sigma 756888-50G UltraPureTM 1 M Tris-HCI Buffer, pH 7.5 Invitrogen 15567027 Zr-89 oxalate Washington University at St. Louis Radionuclide Processing Laboratory Dulbecco’s Phosphate Buffered Saline Sigma D8537 Chloroform Sigma C2432 2-propanol Fisher Scientific A41701 UltaPureTM Glycogen Thermo Fisher Scientific 10814010 iScriptTM cDNA Synthesis Kit BioRad 1708891 iQTM SYBR® Green Supermix BioRad 1708880 RPMI-1640 medium Sigma R8758-1L DMEM- high glucose Sigma D5796-500ML .. MaxPar MCP9 Antibody Labeling Kit, 106Cd Fluidigm Cat # 201106A MaxPar MCP9 Antibody Labeling Kit, 111Cd Fluidigm Cat # 201111A MaxPar MCP9 Antibody Labeling Kit, 112Cd Fluidigm Cat # 201112A MaxPar MCP9 Antibody Labeling Kit, 113Cd Fluidigm Cat # 201113A MaxPar MCP9 Antibody Labeling Kit, 114Cd Fluidigm Cat # 201114A MaxPar MCP9 Antibody Labeling Kit, 116Cd Fluidigm Cat # 201116A MaxPar X8 Antibody Labeling Kit, 146Nd Fluidigm Cat # 201146A MaxPar X8 Antibody Labeling Kit, 149 Sm Fluidigm Cat # 201149A MaxPar X8 Antibody Labeling Kit, 154 Sm Fluidigm Cat # 201154A MaxPar X8 Antibody Labeling Kit, 156 Gd Fluidigm Cat # 201156A MaxPar X8 Antibody Labeling Kit, 162 Dy Fluidigm Cat # 201162A MaxPar X8 Antibody Labeling Kit, 165 Ho Fluidigm Cat # 201165A MaxPar X8 Antibody Labeling Kit, 166 Er Fluidigm Cat # 201166A MaxPar X8 Antibody Labeling Kit, 168Er Fluidigm Cat # 201168A MaxPar X8 Antibody Labeling Kit, 173Yb Fluidigm Cat # 201173A ELISA MAXTM standard set Mouse TNF-a BioLegend 430901 ELISA MAXTM standard set Mouse IL-6 BioLegend 431301 Mouse MIF DuoSet ELISA R&D Systems DY1978 Invitrogen pHrodoTM Green S. aureus BioParticlesTM Phagocytosis Kit for Flow cytometry Thermo Fisher Scientific P535367 Amylase Activity Assay Kit Millipore Sigma MAK009 QIAGEN RNAeasy Kit QIAGEN 74104 Promega Luciferase Assay Systems Luciferase Assay w/ Reporter Lysis buffer Promega E4030 Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1, 4 rxns 10X Genomics PN-1000128 MiSeq Reagent Micro Kit V2 300 cycles Illumina MS-102-2002 NextSeq 500 High Output Kit v2.5 (150 cycles) Illumina 20024907 .. KPC Cell Line Ximbio 153474 Pan02 Cell Line Gift from Dr. Yong Lu at Wake Forest University KPC/FC1242 Luc/GFP clone 3 Gift from Dr. Michael Dwinell, PhD at the Medical College of Wisconsin Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory 000664 Mouse: B6.129S4-Ccr2 tm1I fc/J (CCR2 -/-) The Jackson Laboratory 004999 Mouse: Dectin-1 -/- Bred at the University of Louisville (Saijo et al, 2007) Mouse: NOD.Cg-Prkdcsc id Il2rg tm1Wjl/SzJ (NSG) The Jackson Laboratory 005557 Mouse: B6(Cg)-Tyr c-2J (B6 Albino mice) Gift from Dr. Warawa and bred at the University of Louisville



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